RESEARCH PROJECTS

C_edited_edited_edited.jpg

ELEVATED TEMPERATURE AND MUSCLE DEVELOPMENT OF JUVENILE EPAULETTE SHARKS

Epaulette shark (Hemiscyllium ocellatum) individuals were reared under temperatures 5℃ higher than average known rearing temperatures. Confocal microscopy and transmission electron microscopy (TEM) were used for measures of skeletal muscle development and immunoblotting techniques for expression of muscle growth, oxidative stress, and protein degradation biomarkers to understand potential effects of chronic elevated incubation temperatures on individual shark viability.

GSL+WGA+DAPI Skates 10_17_19.xlef_B42E_1

ELEVATED TEMPERATURE AND PECTORAL FIN STRUCTURE IN THE LITTLE SKATE

Little Skate (Leucoraja erinacea) individuals were reared under temperatures 5℃ higher than ambient rearing temperatures. Pectoral fins were analyzed using confocal microscopy to detect changes in fin morphology and cellular glycosylation and TEM for measures of cellular damage as a result of higher temperatures.

9400_4 cap.jpg

ELEVATED TEMPERATURE EFFECTS ON MUSCLE MORPHOLGOY OF JUVENILE PORT JACKSON SHARKS

Heterodontus portusjacksonii (Port Jackson shark) individuals from Adelaide, South Australia and Jervis Bay, New South Wales were reared at their respective ambient and projected end-of-century (EOC) temperatures from eggs to early juvenile stages. Skeletal muscle was analyzed using confocal microscopy to assess changes in morphology resulting from temperature differences.

1-s2_edited_edited.jpg

HYPOXIA-INDUCED AUTOPHAGY  AND ENVIRONMENTAL ADAPTATION

Hypoxia (oxygen deficiency) is a commonly studied stressor in the biomedical field related to pathological conditions, yet hypoxia is also an environmental stressor. Many marine organisms face and have behavioral or physiological strategies to combat common hypoxia levels, yet the concern for chronic hypoxia is increasing due to potential climate change effects on ocean chemistry. Hypoxia can induce autophagy to protect cells from apoptosis (cell death), and has yet to be studied in the context of potential physiological mechanisms employed by known hypoxia-tolerant marine species. Through the study of a known hypoxia-tolerant marine species, we might be able to further elucidate the extent to which hypoxia-induced autophagy is used and other factors that may be involved in its initiation.

Photo Credit: Mazure and Pouysségur 2010. Current Opinion in Cell Biology